In the spirit of working hard to help others this Labor Day, some of country music's most iconic stars came together and lent their talents to a good cause. Martina McBride, Lady Antebellum and Darius Rucker performed on the MDA Labor Day Telethon this weekend, which directly benefited those with muscular dystrophy in a really big way.

The 46th annual prime-time televised telethon aired on Sept. 4, and was packed to the brim with all-star entertainment. "So glad to be here at the Grand Ole Opry on such a special night," McBride said as she opened the show, adding, "I want to invite you all to make a muscle and make a difference," which enticed the audience to show her their muscles.

McBride delivered a jaw-dropping rendition of 'Somewhere Over the Rainbow,' and during the performances, MDA highlighted four special young people struggling with neuromuscular disease to give the disease a face and make the cause seem more real to viewers who may not be directly affected. Rucker laid down his single, 'I Got Nothin',' while Lady A delivered 'Just a Kiss' to the Opry crowd.

The MDA Labor Day Telethon ran until midnight -- a total of six hours. While host Jerry Lewis, who led the telethon from 1966 until 2010, was absent, the show went on to raise $61.5 million last night alone.

Watch Martina McBride Perform 'Somewhere Over the Rainbow'

Watch Lady Antebellum Perform 'Just a Kiss'